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Fret Peptides, Native Substrates and Receptors, List Biological

Supplier: List Biological Laboratories, Inc.
690B 560 550 540 541 530 542 531 520 521 500A 510A 523 525 526 549 539 528 529
The potent toxicity of both the botulinum neurotoxins and anthrax lethal toxin is due to a zinc-dependent proteolytic activity associated with the toxins.  Measurement of this enzymatic activity provides for both a potentially sensitive and direct means for detection of the toxin, and a method for identifying potential toxin inhibitors using high throughput screening. A highly efficient approach for monitoring enzymatic activity is based on the use of fluorescence resonance energy transfer (FRET) substrates. These fluorogenic peptides contain a donor fluorescent group at one end and a suitable chromogenic acceptor group at the other.  The fluorescence is quenched initially by intramolecular energy transfer between the donor/acceptor pair.  Cleavage of the FRET substrate by the appropriate enzyme releases the fluorophore and full fluorescence is restored.  The increase in fluorescence intensity is directly proportional to the amount of enzyme present.  Enzymatic activity can be monitored continuously by recording the increase in fluorescence intensity with time.  The change in the relative fluorescence units (RFU) as cleavage occurs can be converted to nmoles of cleaved substrate from a standard curve generated using a Calibration Peptide which is the cleaved substrate containing only the N-terminally attached fluorophore.  For Botulinum neurotoxin type A, a Control FRET peptide substrate that is not cleaved by the neurotoxin but contains all remaining non-specific sites in the sequence can be used to screen background cleavage of the substrate that can occur in complex matrices.

There are seven immunologically distinct botulinum neurotoxins, designated types A-G. Each neurotoxin toxin cleaves one of two target proteins critical for synaptic vesicle fusion to the plasma membrane and neurotransmitter release, either SNAP-25 or Synaptobrevin. The latter is also called VAMP-2. Types A, C and E specifically bind and selectively cleave SNAP-25 while types B, D, F and G cleave Synaptobrevin. Type C also cleaves Syntaxin. The enzymatic activity of these toxins can also be measured using the appropriate native substrate and monitoring the generation of the cleaved product on SDS-PAGE gel electrophoresis.

The synaptic vesicle glycoprotein 2c (SV2c) has been shown to be the protein receptor for Botulinum neurotoxin, Type A (BoNT/A) and the luminal domain loop is the specific location of BoNT/A binding.  This 124 amino acid domain of SV2c is available as a GST fusion protein.




 

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Description Size Supplier No. VWR Catalog Number Unit Price Quantity
Calibration Fluorophore for VAMPtide® 540 for Clostridium botulinum Type B Neurotoxin 100 nmoles 549 102946-494 Each (100Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Control Peptide for SNAPtide® 520 substrate for Clostridium botulinum Type A Neurotoxin 200 nmoles 525 102946-594 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Control Peptide for SNAPtide® 521 substrate for Clostridium botulinum Type A Neurotoxin 200 nmoles 526 102946-596 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

GST Synaptobrevin-2 Protein Recombinant Protein Substrate for Clostridium botulinum Type B, D and F 100 µg 510A 102946-586 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

MAPKKide® Peptide Substrate (DABCYL/FITC) for Anthrax Lethal Factor 200 nmoles 531 102946-604 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

MAPKKide® Peptide Substrate (o-Abz/Dnp) for Anthrax Lethal Factor 200 nmoles 530 102946-602 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Receptor for Clostridium botulinum Type A Neurotoxin GST-SV2c luminal domain (U.S. Patent #8,476,024) 100 nmoles 690B 102946-500 Each (100Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

SNAP-25 Recombinant Protein Substrate for Clostridium botulinum Type A and E Neurotoxin 100 µg 500A 102946-584 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

SNAP Etide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type E Neurotoxin 100 nmoles 550 102946-496 Each (100Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

SNAPtide® Peptide Substrate (FITC/DABCYL) for Clostridium botulinum Type A Neurotoxin 200 nmoles 521 102946-590 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

SNAPtide® Peptide Substrate (o-Abz/Dnp) for Clostridium botulinum Type A Neurotoxin 200 nmoles 520 102946-588 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

SNAPtide® Peptide Substrate flP6 (DABCYL/5-IAF) for Clostridium botulinum Type A Neurotoxin 200 nmoles 523 102946-592 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

SYNTAXtide® Peptide Substrate (o-Abz/Dnp) for Clostridium botulinum Type C Neurotoxin 200 nmoles 560 102946-498 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Unquenched Calibration Peptide for MAPKKide® 530 for Anthrax Lethal Factor 50 nmoles 539 102946-606 Each (50Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Unquenched Calibration Peptide for SNAPtide® 520 substrate for Clostridium botulinum Type A Neurotoxin 50 nmoles 529 102946-600 Each (50Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Unquenched Calibration Peptide for SNAPtide® 521 substrate for Clostridium botulinum Type A Neurotoxin 50 nmoles 528 102946-598 Each (50Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

VAMPtide® Peptide Substrate (FITC/DABCYL) for Clostridium botulinum Type B Neurotoxin 200 nmoles 541 102946-610 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

VAMPtide® Peptide Substrate (o-Abz/Dnp) for Clostridium botulinum Type B Neurotoxin 200 nmoles 540 102946-608 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

VAMPtide® Peptide Substrate (PL 150, Pya/Nop) for Clostridium botulinum Type B Neurotoxin 200 nmoles 542 102946-612 Each (200Nmo) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

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SPECIFICATIONS

Protein/Peptide Name Unquenched Calibration Peptide for SNAPtide® 520 substrate for Clostridium botulinum Type A Neurotoxin VAMPtide® Peptide Substrate (PL 150, Pya/Nop) for Clostridium botulinum Type B Neurotoxin Receptor for Clostridium botulinum Type A Neurotoxin GST-SV2c luminal domain (U.S. Patent #8,476,024) Calibration Fluorophore for VAMPtide® 540 for Clostridium botulinum Type B Neurotoxin SNAPtide® Peptide Substrate flP6 (DABCYL/5-IAF) for Clostridium botulinum Type A Neurotoxin VAMPtide® Peptide Substrate (FITC/DABCYL) for Clostridium botulinum Type B Neurotoxin Control Peptide for SNAPtide® 520 substrate for Clostridium botulinum Type A Neurotoxin MAPKKide® Peptide Substrate (DABCYL/FITC) for Anthrax Lethal Factor Unquenched Calibration Peptide for MAPKKide® 530 for Anthrax Lethal Factor Control Peptide for SNAPtide® 521 substrate for Clostridium botulinum Type A Neurotoxin SYNTAXtide® Peptide Substrate (o-Abz/Dnp) for Clostridium botulinum Type C Neurotoxin SNAPtide® Peptide Substrate (o-Abz/Dnp) for Clostridium botulinum Type A Neurotoxin SNAPtide® Peptide Substrate (FITC/DABCYL) for Clostridium botulinum Type A Neurotoxin Unquenched Calibration Peptide for SNAPtide® 521 substrate for Clostridium botulinum Type A Neurotoxin VAMPtide® Peptide Substrate (o-Abz/Dnp) for Clostridium botulinum Type B Neurotoxin SNAP Etide® Peptide Substrate (o-Abz/Dnp) for C. botulinum Type E Neurotoxin Substrate for Clostridium botulinum Type B, D and F MAPKKide® Peptide Substrate (o-Abz/Dnp) for Anthrax Lethal Factor Substrate for Clostridium botulinum Type A and E Neurotoxin
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