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Anti-ZBTB7A Rabbit Polyclonal Antibody

Supplier: Bioss
Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.

Recommended Dilutions: Western Blot: 1:100-1000; IHC-P: 1:100-500; IF(IHC-P): 1:50-200

Type: Primary
Antigen: ZBTB7A
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat



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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 µg BS-10235R 10364-414 Each (100µl) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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Antigen Symbol ZBTB7A
Antigen Name Zinc Finger And BTB Domain Containing 7A
Reactivity Mouse, Rat, Human
Conjugation Unconjugated
Clonality Polyclonal
Antibody Type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
ImmunoChemistry Yes
Antigen Synonyms ZNF857A, LRF, ZBTB7, FBI-1, pokemon, FBI1
Gene ID 51341
Concentration 1 μg/μl
Cross Adsorption No
Purification Purified by Protein A
Form liquid
Storage Buffer Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Storage Temperature Store at -20°C for 12 months
Shipping Temperature 4°C
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