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Donkey Anti-Mouse IgG Polyclonal Antibody (TRITC (Tetramethylrhodamine Isothiocyanate))

Supplier: Immunoreagents
TRITC (Tetramethylrhodamine isothiocyanate) is a popular rhodamine derivative pink in color but emits a red-orange color upon Emission at 580nm. TRITC is frequently paired with FITC in double labeling experiments and conjugated antibodies are often used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. These conjugates are best excited with the 532 nm spectral line of the He-Ne laser and are also recommended as a second color detection reagent in in situ hybridization applications. (Excitation/Emission = 555nm / 580nm Emission Color = Orange to Red (Similar Dyes: Alexa Fluor 555, Cy3, DyLight 550)

Western blot analysis of recombinant human IL-7 (100-11) using rabbit anti-human IL-7 polyclonal antibody and anti-rabbit secondary antibodies. MW of human IL-7 is 17.5 kDa.

Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Conjugation: TRITC (Tetramethylrhodamine Isothiocyanate)
Host: Donkey
Isotype: IgG
Reactivity: Mouse

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Size Supplier No. VWR Catalog Number Unit Price Quantity
0.5 mg IR2877 10014-102 Each (500µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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Antigen Symbol IgG
Antigen Name Immunoglobulin G
Reactivity Mouse
Conjugation TRITC (Tetramethylrhodamine Isothiocyanate)
Clonality Polyclonal
Antibody Type Secondary
Host Donkey
Isotype IgG
Western Blot Yes
ImmunoChemistry Yes
ImmunoPrecipitation Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Concentration 1.0 mg/ml (E 1% at 280 nm = 13.0)
Format Purified IgG
Cross Adsorption Yes
Immunogen Mouse IgG
Purification Affinity purified using solid phaseMouse IgG
Form Lyophilized
Antibody Fragment F(ab')2
Storage Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Storage Temperature 2-8 °C
Shipping Temperature 2-8 °C
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