Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle. The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage. CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma.
Recommended Dilutions: ELISA: 1:10,000-1:20,000; Western Blot: 1-2 ?g/mL; contains 0.02% (w/v) Sodium Azide
Type: Primary
Antigen: CENPO
Clonality: Polyclonal
Clone: 0
Conjugation: Unconjugated
Epitope: Center
Host: Rabbit
Isotype:
Reactivity: