Benzamidine Sepharose 4 Fast Flow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein
- For affinity chromatography
- p-aminobenzamidine (pABA) immobilized on Sepharose 4 Fast Flow matrix gives high productivity, and allows easy scale-up
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant proteins
- Amide coupling method
- Stable in all commonly used aqueous buffers
Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates. Benzamidine Sepharose 4 Fast Flow (high sub) is based on highly cross-linked 4% agarose enabling high flow rates without losing binding capacity
For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.