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NovaTaq™ Hot Start DNA Polymerase, MilliporeSigma

Supplier: MilliporeSigma

NovaTaq™

71091-3 71091-4 71091-4 71091-3

80511-392EA 688.09 USD

CA80511-394 80511-392 CA80511-392 80511-394

NovaTaq™ Hot Start DNA Polymerase, MilliporeSigma

Nucleic Acid Reagents End-point PCR Enzymes and Kits

NovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature.

Highly specific, thereby minimizes the non-specific amplifications
Improved low-copy target amplification
Ambient temperature setup compatible with automation
Target amplification of up to 5 kbp
Ideal for quantitative and high-throughput PCR applications

The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products.

The enzyme must be activated by heat treatment (7 - 10 min at 95 °C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-nucleotide overhangs, suitable for cloning with a varierty of vector kits.

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