Anti-PDYN Guinea Pig Polyclonal Antibody

GTX80766
89265-182EA 663.38 USD
89265-182
Anti-PDYN Guinea Pig Polyclonal Antibody
Guinea Pig polyclonal to proDynorphin antibody

Immunohistochemistry 1:500 (Dilutions listed only as a recommendation. Optimal dilution should be determined by investigator.)Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.

Type: Primary
Antigen: PDYN
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: Guinea Pig
Isotype:
Reactivity: Rat
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