WB: Use at a dilution of 1/1000. A starting dilution of 1/5000 is recommended for chemiluminescent substrates. By immunoblotting against the reduced protein, the antibody identifies bands at 30 kDa and 18 kDa (zymogen and active MMP26). MMP26 (matrilysin 2) does not appear to be produced by most normal quiescent cells, but the treatment of many cell types with the phorbol ester TPA or IL1 stimulates production of MMP26. Because of the low protein levels produced (pg/ml) concentration of cell culture media is often required to visualize the bands by immunoblotting. Note: Higher antibody dilutions may be necessary for non-human samples. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: MMP26
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human