Anti-DNAL1 Goat Polyclonal Antibody

Supplier: Genetex

89317-234EA 415.09 USD
Anti-DNAL1 Goat Polyclonal Antibody
Goat polyclonal to IL4

ELISA: Use at a concentration of 0.5 - 1 ?g/ml, this detects 0.3 ng/well of rhIL4. Neut: Use at a concentration of 0.004 - 0.012 ?g/ml. WB: Use at a concentration of 0.1 - 0.2 ?g/ml, this detects rhIL2 at 2 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 20 kDa. Anti-Human IL4 is tested for its ability to neutralize the bioactivity of rhIL4 in a cell proliferation assay using a human factor-dependent cell line, TF1. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhIL4 that is present at a concentration just high enough to elicit a maximum response. In this bioassay, rhIL4 was preincubated with various dilutions of the antibody for 1 hour at 37 °C in a 96-well microtiter plate. TF1 cells were added to each well. The total volume of 100 ul, containing antibody, rhIL4 at 0.5 ng/ml and cells at 1 x 10^5 cells/ml, was incubated for 48 hours at 37 °C in a 5% CO2 humidified incubator and then pulsed for the last 4 hours with ^3H-thymidine. Cells were harvested onto glass filters and the ^3H-thymidine incorporation into DNA was measured. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: DNAL1
Clonality: Polyclonal
Host: Goat
Reactivity: Human, Mouse, Rat
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