Anti-FAHD2A Mouse Monoclonal Antibody [clone: 22221.311]

Supplier: Genetex

GTX10503
89315-998EA 358.02 USD
89315-998
Anti-FAHD2A Mouse Monoclonal Antibody [clone: 22221.311]
Antibodies
Mouse monoclonal [22221.311] to TNFRSF1B

ELISA: Use at a concentration of 0.5 - 1 ?g/ml, this concentration is determined to detect recombinant human sTNF RII to a limit of 1.25 ng/well. Neut: Use at a concentration of 0.5 - 1 ?g/ml, this concentration will block 50% of the bioactivity due to 0.3 ?g/ml recombinant human sTNF RII, in the presence of 0.25 ng/ml TNF-alpha in a crystal violet cytolytic assay using mouse L929 cells. WB: Use at a concentration of 1 - 2 ?g/ml, this concentration is determined using recombinant human sTNF RII at 50 ng/lane under non-reducing conditions. Predicted molecular weight: 75 kDa. Anti-Human sTNF RII is tested for its ability to neutralize the biological effect of sTNF-RII but not sTNF RI in a cytolytic assay using mouse L929 cells, in the presence of TNF-alpha. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of the soluble receptor activity on a responsive cell line, when the soluble receptor is present at a concentration just high enough to elicit a maximum response. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: TNFRSF1B
Clonality: Monoclonal
Clone: 22221.311
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Human
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