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Anti-HSP60 IgG/A/M (human), ELISA Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
EKS-650
Most comprehensive portfolio of heat shock protein (chaperone) products commercially available.

For the quantitative determination of anti-HSP60 IgG/A/M autoantibodies in human serum.

This assay allows for reproducible, accurate and precise determination of IgG , IgA and IgM antibodies (total) to human Hsp60 in serum. The ELISA kit uses recombinant human Hsp60 bound to the wells of the plate to bind anti-human Hsp60 antibodies present in human serum. The captured anti-human Hsp60 antibodies are detected with a HRP conjugated goat polyclonal antibody specific for human IgG, IgA and IgM molecules. The assay is developed with tetramethylbenzidine substrate pro ducing a blue color in proportion to the amount of captured anti -human Hsp60 antibodies. The color development is stopped with acid stop solution which converts the endpoint color to yellow. The intensity of the color is measured in a microplate reader at 450 nm.

Ordering information: Contains Microtiter plate, Standard, Sample diluent 1, Wash buffer concentrate, HRP Conjugate, TMB Substrate, Stop solution 2.




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No. of Wells Supplier No. VWR Catalog Number Unit Price Quantity
96 Wells ADI-EKS-650 89141-856 Each Retrieving

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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SPECIFICATIONS

Sensitivity 2.88 ng/ml (range 7.81 - 250 ng/ml)
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