M9 CA Medium, Quality Biological

Supplier: Quality Biological
340-017-101
10128-268EA 65.85 USD
10128-268
M9 CA Medium, Quality Biological
Media for Microbiology Prepared Media for Microbiology
M9 CA Media contains the essentials for bacterial species to grow. Important components of M9 CA medium are the carbon source from glucose, a nitrogen source from ammonia chloride, and electrolytes from sodium, chloride, potassium, calcium, phosphorus and magnesium salts in the M9 solution.

  • Glucose as a carbon source and casamino acids as a nitrogen source.

M9 CA Media is often used to define if a particular microbial species is a heterotroph, namely an organism that does not have any nutritional requirements beyond core sources of carbon (sugars) and nitrogen (to synthesize amino acids and nucleic acid bases).

Auxotrophs, or organisms with nutritional requirements will not be able to grow on minimal media. M9 CA medium supports the growth of recombinant strains of E. coli and wild-type strains of E. coli. Advantageously, M9 medium is inexpensive, low auto-fluorescence (when excited at 488 nm) and low absorbance M9 CA.

M9 Medium is used for the following applications: propagation of E. coli strains, maintaining positive selective pressure on recombinant strains of E. coli with plasmids coding for the ability to produce essential substances such as amino acids or vitamin, and maintaining stocks of F’-containing bacteria for use with M13 bacteriophage.

Recommendation
Supplementing M9 with magnesium and calcium will increase the growth of recombinant E. coli. Also, M9 can be supplemented with specific amino acids or other metabolites to allow for selection of specific auxotrophs

Sterile Filtered
UNSPSC: 41106200
Harmonization Code: 3822.00.5090
ECCN: EAR99

Certifications: Manufactured under a cGMP compliant quality management system in an ISO 9001:2015 certified facility.

Caution: Research Use Only
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