Neutralization solution
Synonyms:
DNA Neutralizing solution
, Neutralization solution
, Neutralisation solution
, DNA Neutralising solution
DNA Neutralizing Solution used to neutralize fractionated DNA contained in an electrophoresis gel after denaturization and prior to transfer to nitrocellulose or nylon membrane
- Sterile
- Shelf life 36 months
When utilized in conjunction with a depurination solution, treatment with the denaturing solution results in the hydrolysis of the phosphodiester backbone at the sites of depurination. This allows for a more efficient transfer of large DNA in neutral Southern transfers. Following DNA denaturation, the gel must be neutralized by soaking in DNA Neutralization Solution.
Caution: Research Use Only
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...