Coomassie® brilliant blue R-250, dark powder for electrophoresis

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

Synonyms: Brillant blue RBrilliant indocyanine 6BBrilliant Blue RServa Blue RHydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulfonatobenzyl)amino]benzhydrylene]cyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulfonatobenzyl)ammonium monosodium saltCoomassie Blue R 250Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]benzhydrylene]cyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium monosodium saltAcid Blue 83Brilliant Cyanine 6B

04821616 04821636
IC821616EA 89.09 USD
IC821616 IC821636
Coomassie® brilliant blue R-250, dark powder for electrophoresis
Coomassie® Brilliant Blue R-250

Coomassie® Brilliant Blue R-250 is a fast acting, sensitive dye, which can be used on SDS gels, IEF gels, and standard gels.


  • For Detection of Protein Bands Following Electrophoresis


Brilliant Blue R can be used for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates.


Soluble in water (10 mg/ml), 20 mg/ml in methyl cellosolve, ethanol (10 mg/ml), methanol.


Caution: Research Use Only (RUO).

Formula: C₄₅H₄₄N₃NaO₇S₂
MW: 825.98 g/mol
Storage Temperature: Ambient
MDL Number: MFCD00041762
CAS Number: 6104-59-2

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Specification Test Results

Identity Test Passes
λmax (ethanol) 585 ± 5 nm
Extinction Coefficient E1%(587nm) = 785 (methanol)

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