DEAE-Dextran chloride form, off-white powderSupplier: MP BIOMEDICALS (FKA ICN BIOMED
DEAE-Dextran, a polycationic diethylaminoethyl ether of dextran is a stabilizing molecule and transfection reagent. The diethylaminoethyl groups are linked to glucose residues by ether linkages.
Prepared from dextran MW: 500 000
Nitrogen: ~ 3,2%
DEAE-Dextran is used in the development of sustained release protein delivery and nucleic acid transfection systems. The use of DEAE-Dextran as an efficient adjuvant in vaccine production is well established. For instance, DEAE-Dextran has been used for immunization of guinea pigs and swine with inactivated foot-and-mouth disease and rhesus monkeys with inactivated Venezulean equine encephalomyelitis virus, IVEE. The mechanism of action of DEAE-Dextran is not clear. The effect on the humoral response of rhesus monkeys to IVEE vaccine results in a typical IgM-IgG response. It has been speculated that DEAE-Dextran may cause a stimulation of the helper T-cell function in antibody synthesis.
Soluble in water (100 mg/ml) and dilute salt solutions. Solutions can be sterilized by autoclaving even at low pH. Autoclaving can be done at 110 to 115 °C. For 20% (w/v) or less solutions autoclave for approximately 30 minutes; for solutions above 20%, autoclave for approximately 15 minutes. Cool slowly.
Specification Test Results
|Nitrogen||3 - 4%|
|pH||5.0 - 7.0|
|Loss on Drying||≤10%|
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