Auramine O, dark yellow powder C.I. 41000

Synonyms: 4,4'-(Iminomethylene)bis(N,N-dimethylaniline) hydrochloridePyoctanunum aureum4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloridePyoktanin YellowBasic Yellow 2Canary Yellow

0219506450
IC19506450EA 105.02 USD
IC19506450
Auramine O, dark yellow powder C.I. 41000
Auramine O

Auramine O is used for acid resistant bacteria in sputum or paraffin.


It is used with rhodamine B, causing acid-fast organisms to fluoresce. Used with acridine orange, showing acid-fast organisms and fungi, mycobacteria. (Auramine O is reportedly specific for mycolic acid). It is used as a Schiff reagent, causing DNA in nuclei to fluoresce yellow-green and is used with safranin O chloride to stain spores in bacterial cultures.


Soluble in ethanol (1 mg/ml), water (10 mg/ml).

Formula: C₁₇H₂₂ClN₃
MW: 303.84 g/mol
MDL Number: MFCD00012484
CAS Number: 2465-27-2
UN: 2811
ADR: 6.1,III

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Specification Test Results

Identity Test Passes
UV/VIS: λmax (water) 432±3 nm

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