ABTS (Diammonium 2,2'-azinobis[3-ethyl-2,3-dihydrobenzothiazole-6-sulfonate]), light green powderSupplier: MP BIOMEDICALS (FKA ICN BIOMED
, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt
, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt
2,2-Azino-bis-(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) has been used as a chromogenic substrate for horseradish peroxidase (HRP), both in general activity assays and in ELISA applications. Activity of HRP using ABTS appears about four-fold higher than using pyrogallol. It is mainly used as a substrate in sensitive peroxidase assays.
2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
- Presentation: Light Green Powder
- Extinction Coefficient (E1%): EmM(342 nM) > 37.4; EmM(225 nM) > 48.15; EmM(414 nM) = 36 (Lit.)
- Soluble in water (5% w/v - clear, pale green solution).
- Storage temperature: Store at +4 °C. Store Under Nitrogen. Protect from light.
MW: 548.68 g/mol
Storage Temperature: Refrigerator
CAS Number: 30931-67-0
Specification Test Results
|FTIR||Conforms to Standard|
|UV/VIS: I max (water)||340 - 342, 224 - 228 nm|
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