Leishman's eosin-methylene blue, greenish-black powder
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Synonyms:
Eosin and methylene blue Leishman
, Eosin-Methylene blue dye Leishman
, Leishman stain
, Leishman's stain (eosin methylene blue compound)
, Leishman's stain
Leishman's stain, also Leishman stain, is used in microscopy for staining blood smears. It provides excellent stain quality. It is based on a methanolic mixture of "polychromed" methylene blue (i.e. demethylated into various azures) and eosin.
The methanolic stock solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step. If a working solution is made by dilution with an aqueous buffer the resulting mixture is very unstable and cannot be used for long. Leishman stain is a Romanowsky-type dye, similar to Wright stain.
Leishman's Stain is used generally in hematology and tropical medicine to differentiate and identify leucocytes, malarial parasites and trypanosomes. .
Soluble in water (4 mg/ml), ethanol (4 mg/ml), in 0,1% methanol (clear, blue soln with fluorescence) or methyl cellosolve (20 mg/ml).
Storage Temperature:
Ambient |
MDL Number:
MFCD00131498 CAS Number: 12627-53-1 |
Specification Test Results
Identity Test | Passes |
l max (methanol) | 645 ± 5 nm |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...