Concanavalin A (from Jack Bean)Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Pure Canavalia ensiformis lectin (Con A) from Jackbean. Isolated by affinity chromatography on cross-linked dextran. Con A exists as a dimer below pH 5.0 and and at pH >7 it exists as a tetramer. Con-A is not a glycoprotein. The monomeric molecular weight of Con-A is 25,500. Con-A does not contain cysteine residues. Unlike most other lectins, Con-A is a metalloprotein and requires a transition metal ion, such as manganese, plus calcium ions for binding.
Lectin are used in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Con-A has been used to elucidate structural changes in the membrane surface of transformed cells. Con-A conjugates of antitumor drugs have been used in drug delivery systems in cultured cells.
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6. Con-A binds specifically to mannosyl and glucosyl residues of polysaccharides and glycoproteins. Unmodified hydroxyl groups at the C3, C4 and C6 positions of D-glucopyranosyl or D-mannopyranosyl rings may be essential for binding. Each subunit of Con-A contains one calcium ion and one manganese ion. Removal of these cations by dialysis under acidic conditions abolishes the carbohydrate-binding activity.
- Presentation: White Shiny Powder
- Isoelectric point (pl): 4.5, 4.7, 5.0-5.1, and 5.4-5.5 (several isoforms)(Lit.)
- Inhibitory Carbohydrate: Methyl-a-D-Mannopyranoside >> a-D-Mannose >> a-D-Glucose
- Carbohydrate Specificity: a-Mannose, a-Glucose, Branched Mannose.
- Soluble in water (10 mg/ mL-slightly hazy, colorless solution).
- Storage temperature: -20 °C
CAS Number: 11028-71-0
Specification Test Results
|Source||Canavalia ensiformis seeds (jack beans)|