(2-Nitrophenyl)-β-D-galactopyranoside (ONPG), crystalline

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

Synonyms: ONPG(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(2-nitrophenoxy)tetrahydro-2H-pyran-3,4,5-triolo-Nitrophenyl-β-D-galactosideO-Nitrophenyl-Β-D-Galactopyranoside

0210247305
IC10247305EA 162.61 USD
IC10247305
(2-Nitrophenyl)-β-D-galactopyranoside (ONPG), crystalline
(2-Nitrophenyl) β-D-galactopyranoside

o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.


β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.


Soluble in water (10 mg/mL- clear, colorless solution; 15 mg/mL - clear, faint yellow solution; 20 mg/mL with heat - clear, yellowish solution).


Caution: Research Use Only (RUO).

Formula: C₁₂H₁₅NO₈
MW: 301.25 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00063255
CAS Number: 369-07-3

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Specification Test Results

Identity test Passes
FTIR Conforms to standard
Optical Rotation (c=1, H₂O) –66° ± 7°
Melting Point 180 - 195 °C
UV/VIS: λmax (H₂O) 262 ±3 nm

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