β-Dihydronicotinamide adenine dinucleotide disodium salt (NADH-Na₂, reduced form) ≥98%

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

Synonyms: ((2R,3S,4R,5R)-5-(6-Aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl)methoxy-((((2R,3S,4R,5R)-5-(3-carbamoyl-4H-pyridin-1-yl)-3,4-dihydroxy-oxolan-2-yl)methoxy)hydroxyphosphoryl)oxyphosphinic acid disodium saltNicotinamide adenine dinucleotide reduced, disodium salt (reduced form)

0210116810 0210116801 02101168.1 0210116805
IC10116810EA 1104.61 USD
IC10116810 IC10116801 IC101168.1 IC10116805
β-Dihydronicotinamide adenine dinucleotide disodium salt (NADH-Na₂, reduced form) ≥98%
β-Dihydronicotinamide adenine dinucleotide disodium salt (NADH-Na2, reduced form)

White solid, soluble in water or aqueous buffers (50 mg/ml); soluble in 0.01 N Sodium hydroxide (100 mg/mL - clear to slightly hazy, yellowish solution).


Fluorescent Properties: Excitation Wavelength = 340 nm ; Emission Wavelength = 460 nm (Lit.)


β-NADH, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. β-NAD+ is a carrier for hydride ion, forming β-NADH. The hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as, malic dehydrogenase and lactic dehydrogenase. These enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to β-NAD+, forming the reduced product, β-NADH. Unlike β-NAD+, which has no absorbance at 340 nm, β-NADH absorbs at 340 nm. The increase in absorbance (with the formation of β-NADH) or the decrease in absorbance (with the formation β-NAD+) is the basis for measurement of activity of many enzymes at 340 nm.


β-Nicotinamide adenine dinucleotide (NAD+) and β-Nicotinamide adenine dinucleotide, reduced (NADH) comprise a coenzyme redox pair (NAD+:NADH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. In addition to its redox function, NAD+/NADH is a donor of ADP-ribose units in ADP-ribosylaton (ADP-ribosyltransferases; poly(ADP-ribose) polymerases ) reactions and a precursor of cyclic ADP-ribose (ADP-ribosyl cyclases). Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NADH as a cofactor and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. β-NADH is fluorescent; whereas, β-NAD+ is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10^ -7 M. Discussion of optimizing the fluorescence intensity and identification of interfering substances has been reported.


NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, beta-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease. As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.


Store at −0 °C. Store Desiccated. Store Under Nitrogen. Protect from light


Certifications: pH: 7,5 - 9,5 (10% aq soln)

Formula: C₂₁H₂₇N₇Na₂O₁₄P₂
MW: 709.41 g/mol
MDL Number: MFCD00036200
CAS Number: 606-68-8

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Specification Test Results

Form White Powder
Storage Store at -0 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
pH 7.5 - 9.5 (10% aq soln)

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