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Anti-RAG2 Rabbit Polyclonal Antibody

Supplier: Proteintech
Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3

Western Blot:A375 Cells, 1:200-1:1000; IHC: Human Lymphoma Tissue, 1:20-1:200; IF: Hela Cells, 1:10-1:100; FC: HeLa Cells, N/A

Type: Primary
Antigen: RAG2
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse

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Size Supplier No. VWR Catalog Number Unit Price Quantity
150 µL 11825-1-AP 10092-944 Each (150µl) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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Antigen Symbol RAG2
Antigen Name Recombination Activating Gene 2 antigen
Reactivity Mouse, Human
Conjugation Unconjugated
Clonality Polyclonal
Antibody Type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
ImmunoChemistry Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen Synonyms RAG2_HUMAN, Recombination activating gene 2, VDJ recombination-activating protein 2., RAG-2, VDJ recombination activating protein 2, RAG2, RAG 2
Immunogen Recombinant Protein
Purification Antigen affinity Purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Shipping Temperature Store at -20°C
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