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Anti-LIN28 Rabbit Polyclonal Antibody

Supplier: Proteintech
LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin.

Western Blot:K-562 Cells, 1:200-1:2000; IHC: Human Prostate Cancer Tissue, 1:20-1:200; IP:K-562 Cells, 1:200-1:2000; IF: Human embronic stem Cells, 1:20-1:200

Type: Primary
Antigen: LIN28
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat



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Size Supplier No. VWR Catalog Number Unit Price Quantity
150 µL 11724-1-AP 10089-634 Each (150µl) Retrieving Restricted
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SPECIFICATIONS

Antigen Symbol LIN28
Antigen Name Lin-28 homolog A
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Clonality Polyclonal
Antibody Type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
ELISA Yes
ImmunoChemistry Yes
ImmunoPrecipitation Yes
ImmunoFluorescence Yes
Antigen Synonyms Lin-28 homolog A (C. elegans), Zinc finger CCHC domain-containing protein 1, LIN28A, CSDD1, ZCCHC1, RNA-binding proteináLIN-28
Immunogen Recombinant Protein
Purification Antigen affinity Purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Shipping Temperature Store at -20°C
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