The E.Z.N.A
Samples are homogenized and lysed in a high salt buffer containing CTAB and digested with proteinase. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted and BL buffer is added to provide appropriate binding conditions. The sample is then transferred to the HiBind® DNA Maxi Column, where the genomic DNA binds to the membrane and salt and other contaminants are efficiently washed way. High-quality genomic DNA is eluted with elution buffer or water. Purified DNA is suitable for most downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.
High Performance (HP) Tissue DNA Maxi Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from tissue samples up to 2 grams in size. The specially formulated buffer system ensures the optimal lysis of tissues rich in fat, polysaccharides, and fibers such as brain, adipose, and muscle. This kit can also isolate DNA from molluscs, insects, arthropods, roundworms, flatworms, and other invertebrate tissue samples rich in mucopolysaccharides. The procedure relies on the well established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek’s HiBind® matrix.
