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One Step RT-PCR Master Mix Kit, MilliporeSigma

Supplier: MilliporeSigma
The One Step RT-PCR Master Mix Kit allows for rapid analysis of gene expression from tissues and cells

The rTth DNA polymerase is provided in a 2X master mix with an antibody or antibody-mediated hot start, optimized buffer and ultrapure deoxynucleotides. The antibody-mediated hot start enhances specificity of both reverse transcription and PCR. This kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction.

The kit can replace methods for detecting and quantifying gene expression such as Northern blots, in situ hybridization, dot blots, S nuclease assays and conventional two-step RT-PCR. The Once Step RT-PCR Master Mix kit utilizes recombinant Thermus thermophilus (rTth) DNA polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent RNA polymerase.



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Description Size Supplier No. VWR Catalog Number Unit Price Quantity
One Step RT-PCR Master Mix Kit 50 rxns 71978-3 95042-702 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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