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FGF acidic Recombinant Protein, ProSci Inc

Supplier: Prosci
40-159 40-588 40-543 40-493
FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant murine FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues.

Store in working aliquots at -20°C. Avoid freeze/thaw cycles.




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Size Supplier No. VWR Catalog Number Unit Price Quantity
0.01 mg 40-159 10072-038 Each (10µG) Retrieving

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

0.01 mg 40-493 10072-592 Each (10µG) Retrieving

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

0.01 mg 40-543 10072-178 Each (10µG) Retrieving

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

0.05 mg 40-588 10072-204 Each (50µG) Retrieving

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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SPECIFICATIONS

Protein/Peptide Name FGF Acidic FGF acidic FGF Acidic
Protein Synonyms Fgf-1, Fam, Fibroblast growth factor 1, Acidic fibroblast growth factor, Dffrx, Fgfa, FGF-1 Dffrx, Acidic fibroblast growth factor, Fam, Fibroblast growth factor 1, Fgfa, Fgf-1, FGF-1 Fgf-1, Fgfa, Acidic fibroblast growth factor, HBGF1, HBGF-1, FGF-1, Fibroblast growth factor 1 Fgfa, Fibroblast growth factor 1, Fam, Acidic fibroblast growth factor, Fgf-1, FGF-1, Dffrx
Protein/Peptide Type Recombinant
Species Human Mouse Rat Human
Source E. coli
Purity >95%
Formulation Lyophilized
Biological Activity Assay 1: The ED50 was determined by the dose - dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/mL, corresponding to a specific activity of ≥ 1 x 10<sup>5</sup> units/mg.<br><br>Assay 2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.5 ng/mL in the presence of 10 μg/mL heparin, corresponding to a specific activity of ≥ 2 x 10<sup>6</sup> units/mg. Assay 1: The ED50 was determined by the dose - dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is ≤ 0.5 ng/mL corresponding to a specific activity of ≥ 2 x 10<sup>6</sup> units/mg.<br><br>Assay 2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.2 ng/mL in the presence of 10 μg/mL heparin, corresponding to a specific activity of ≥ 5 x 10<sup>6</sup> units/mg. Biological activity was determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is ≤ 0.1 ng/mL, in the presence of 10 μg/mL heparin, corresponding to a specific activity of ≥ 1 x 107 units/mg. Endotoxin level is less than 0.1 ng per μg (1EU/μg). Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is = 10 ng/mL corresponding to a specific activity of = 1 x 10<sup>5</sup> units/mg.<br><br> Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is = 0.5 ng/mL in the presence of 10 μg/mL heparin, corresponding to a specific activity of = 2 x 10<sup>6</sup> units/mg.
Protein Type Recombinant
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