Reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
FAS antibody can be used in Western Blot at 1 - 2 ?g/mL, immunohistochemistry starting at 10 ?g/mL, and immunoprecipitation starting at 300 - 500 µg/1E6 cells.
Ion Exchange Chromatography
20 mM Tris/HCl, 15 mM sodium azide, approx., pH 7.4.
Store FAS antibody at 4 °C or -20 °C. As with all antibodies avoid freeze/thaw cycles.
Type: Primary
Antigen: FAS
Clonality: Monoclonal
Clone: [LT95]
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human