Anti-LEFTY2 Mouse Monoclonal Antibody [clone: [7C5G1H6H10]]Supplier: Prosci
10079-384EA 582.9 USD10079-384
Anti-LEFTY2 Mouse Monoclonal Antibody [clone: [7C5G1H6H10]]
During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 kDa) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.
LEFTY2 antibody can be used in immunohistochemistry starting at 2.5 ?g/mL.
Protein A Column
0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, 0.01% sodium azide.
Store LEFTY2 antibody at 4 °C or -20 °C. As with all antibodies avoid freeze/thaw cycles.