Anti-CASP3 Mouse Monoclonal Antibody [clone: [31A1067 ]]

Supplier: Prosci

49-758
10079-302EA 561.89 USD InStock
10079-302
Anti-CASP3 Mouse Monoclonal Antibody [clone: [31A1067 ]]
Antibodies
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32- kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc.. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17- kDa and a small subunit of 12- kDa. Caspase 3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase 3 proteolytically cleaves and activates other proteins. Caspase 3 has been associated with apoptosis.

Caspase-3 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 ?g/mL.

Protein G Column

PBS, 0.05% BSA, 0.05% sodium azide.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: CASP3
Clonality: Monoclonal
Clone: [31A1067 ]
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG
Reactivity: Human, Mouse
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