Anti-COL3A1 Rabbit Polyclonal Antibody

Supplier: Prosci

49-568
10076-428EA 582.9 USD
10076-428
Anti-COL3A1 Rabbit Polyclonal Antibody
Antibodies
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.

Collagen III antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 5 ?g/mL.

0.125 M sodium borate, 0.075 M NaCl, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: COL3A1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Bovine, Human
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