Anti-NOTCH1 Rabbit Polyclonal Antibody

Supplier: Prosci

49-457
10078-982EA 582.9 USD
10078-982
Anti-NOTCH1 Rabbit Polyclonal Antibody
Antibodies
Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

NOTCH1 antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 5 ?g/mL.

Antiserum

0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, 0.01% sodium azide.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: NOTCH1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human
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