Anti-CUL3 Rabbit Polyclonal AntibodySupplier: Prosci
10078-908EA 582.9 USD10078-908
Anti-CUL3 Rabbit Polyclonal Antibody
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.
Cullin 3 antibody can be used in ELISA starting at 1:1000, and immunohistochemistry starting at 10 ?g/mL.
Delipidation and Defibrination
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.