ELISA: Antibody detection limit dilution 1:32,000. Approximately 150 kDa band observed in human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112 kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272 (44) :28081-8; PMID: 9346962). Recommended concentration: 1-3 ?g/mL. an additional band of 55-60 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this antibody is used for other assays than Western Blot.
SIPA1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: SIPA1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Goat
Isotype:
Reactivity: Human