ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 26 kDa band in lysates of human brain, of T cell line MOLT4, of myeloid erythroblast cell line K562 and of EBV-immortilized lymphoblastoid after 1 ?g/mL antibody staining.1. The 26 kDa band was successfully blocked by incubation with the immunizing peptide. Also,. Western Blot: LRP5 antibody has been successfully used in WB assays with rabbit tissue lysate (whole bone; amount loaded: 20 ?g; antibody dilution 1:200; incubation time of primary antibody: 2 hours; milk (5%) as blocking agent for 1.5 hours; sewcondary antibody: bovine anti-goat with incubation time of 2 hours). Rabbit Bone samples were used and probed at the same time with GAPDH as a loading control. Specific bands of 51 kDa (LRP5) and 37 kDa (GAPDH) were detected. Antibody buffer was 1% NFDM in TBS. (Information was provided by a customer). Immunohistochemistry: In paraffin embedded human pancreas shows staining of cytoplasm in some cells of the islets of Langerhans. Recommended concentration, 1-2 ?g/mL.
LRP5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: LRP5
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Goat
Isotype:
Reactivity: Human