ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 80 kDa band in human brain (cerebellum, hippocampus, frontal cortex) lysates after 0.3 ?g/mL antibody staining. The 80 kDa band was successfully blocked by incubation with the immunizing peptide. Approximately 290 kDa band observed in lysates of mouse fybroblasts with a genetic construct encoding siRNA. The observed band is knocked down by two different siRNA molecules. Calculated MW of 231 kDa according to NP_054878.3).The Ponceau red demonstrates proper protein loading. Recommended concentration: 0.5-2 ?g/mL.
HYPB antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: SETD2
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Goat
Isotype:
Reactivity: Mouse