ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 200 kDa band observed in Jurkat lysate (calculated MW of 215 kDa according to NP_000250.3). Recommended concentration: 1-3 ?g/mL. Immunofluorescence: Transverse sections of mouse skeletal muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, Proc Natl Acad Sci U S A. 2010 Feb 2;107 (5) :2031-6; PMID: 20133847). Recommended concentration: 1-3 3 ?g/mL.
MYO5A antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: MYO5A
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Goat
Isotype:
Reactivity: Human, Mouse