Anti-IL33 Rabbit Polyclonal Antibody

Supplier: Prosci

10098-052EA 405.8 USD InStock
Anti-IL33 Rabbit Polyclonal Antibody
ELISA:<br>Sandwich:<br>To detect hIL-33 by sandwich ELISA (using 100?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IL-33 (38-193) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-33. <br><br>Western Blot:<br><br>To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.<br><br>Neutralization:<br><br>To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/mL), a concentration of 0.2-0.4 ?g/mL of this antibody is required.<br><br>Immunohistochemistry:<br><br>This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 mg/mL-0.25 mg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: IL33
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Reactivity: Human
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