ELISA:<br>Sandwich:<br>In a sandwich ELISA (assuming 100?L/well), a concentration of 14.0-16.0 ?g/mL of this antibody will detect at least 1000 pg/mL of recombinant rat IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-rat IL-2 (38-205) as the detection antibody at a concentration of at least 2.0-4.0 ?g/mL. <br><br>Western Blot:<br><br>To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ?g/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.
Type: Primary
Antigen: IL2
Clonality: Monoclonal
Clone: 2G7-25-3
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Rat