Anti-IL2 Mouse Monoclonal Antibody

Supplier: Prosci

10098-044EA 291.09 USD
Anti-IL2 Mouse Monoclonal Antibody
ELISA:<br>Sandwich:<br>In a sandwich ELISA (assuming 100?L/well), a concentration of 4.0-8.0 ?g/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 ?g/mL. <br><br>Western Blot:<br><br>To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.<br><br>Neutralization:<br><br>To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 ?g/mL of this antibody is required.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: IL2
Clonality: Monoclonal
Clone: 5B9-2-1
Conjugation: Unconjugated
Host: Rat
Reactivity: Mouse
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