Anti-IL1RN Rabbit Polyclonal Antibody

Supplier: ProSci

10098-032EA 455.29 USD
Anti-IL1RN Rabbit Polyclonal Antibody
ELISA:<br>Sandwich:<br>To detect Human IL-1RA by sandwich ELISA (using 100?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IL-1RA (38-139) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA. <br><br>Western Blot:<br><br>To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.<br><br>Neutralization:<br><br>To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-1RA (50.0 ng/mL) and Human IL-1a(50.0 pg/mL), a concentration of 2.5-3.7 ?g/mL of this antibody is required.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: IL1RN
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Reactivity: Human
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