Anti-IGFBP5 Rabbit Polyclonal Antibody

Supplier: Prosci

10098-026EA 420.98 USD
Anti-IGFBP5 Rabbit Polyclonal Antibody
ELISA:<br>Sandwich:<br>To detect hIGF-BP5 by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IGF-BP5 (38-154) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5. <br><br>Western Blot:<br><br>: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.<br><br>Immunohistochemistry:<br><br>This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2.0 mg/mL overnight at 4°C followed by a fluorescent labeled secondary antibody.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: IGFBP5
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Reactivity: Human
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