Anti-Csf2 Rabbit Polyclonal Antibody (Biotin)

Supplier: Prosci

38-284
10098-018EA 420.98 USD
10098-018
Anti-Csf2 Rabbit Polyclonal Antibody (Biotin)
Antibodies
GM-CSF is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors.

ELISA:<br>Direct:<br>To detect Rat GM-CSF by direct ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF. <br><br>Sandwich<br>To detect Rat GM-CSF by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Rat GM-CSF (38-273) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.<br><br>Western Blot:<br><br>To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: Csf2
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Rabbit
Isotype:
Reactivity: Rat
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