Anti-SERPINB5 Rabbit Polyclonal Antibody (Biotin)

Supplier: ProSci
38-278
10098-006EA 576.13 USD
10098-006
Anti-SERPINB5 Rabbit Polyclonal Antibody (Biotin)
Antibodies
Maspin was discovered as a mammary tumor suppressor that is expressed in normal mammary epithelium but lost in most breast cancer cell lines. While maspin is related to the serpin family of serine protease inhibitors, it may not function as a protease inhibitor. It plays an essential role in embryonic development through critical roles in cell adhesion. While the precise mechanism of maspin signaling is unclear, the tumor suppressing activity of maspin has been attributed to its ability to inhibit cell invasion/metastasis and angiogenesis, while promoting apoptosis. Nuclear translocation of active IKKbeta has been shown to repress maspin transcription and promote prostate cancer metastasis.

ELISA:<br>Direct:<br>To detect hMaspin by direct ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin. Sandwich:To detect hMaspin by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human Maspin (38-244) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin.<br><br>Western Blot:<br><br>To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: SERPINB5
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human
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