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Anti-CXCL10 Mouse Monoclonal Antibody [clone: 3.3-1G1-2G7-2F6]

Supplier: ProSci

Sandwich ELISA: assuming 100 µl/well, a concentration of 1.0-2.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IP-10 when used withanti-Human IP-10 (XP-5203Bt) as the detection antibody at a concentration of approximately 0.25 µg/ml.

For WB, antibody can be used at at a concentration of 0.25-0.50 µg/ml (non-reducing) or at a concentration of 1.0-2.0 µg/ml (reducing). The detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.

For WB, antibody can be used at at a concentration of 0.25-0.50 µg/ml (non-reducing) or at a concentration of 1.0-2.0 µg/ml (reducing). The detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.

Sandwich ELISA: assuming 100 µl/well, a concentration of 1.0-2.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IP-10 when used withanti-Human IP-10 (XP-5203Bt) as the detection antibody at a concentration of approximately 0.25 µg/ml.

For WB, antibody can be used at at a concentration of 0.25-0.50 µg/ml (non-reducing) or at a concentration of 1.0-2.0 µg/ml (reducing). The detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.

For WB, antibody can be used at at a concentration of 0.25-0.50 µg/ml (non-reducing) or at a concentration of 1.0-2.0 µg/ml (reducing). The detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.

38-267

10097-984EA 531.34 USD

10097-984

Anti-CXCL10 Mouse Monoclonal Antibody [clone: 3.3-1G1-2G7-2F6]

Antibodies

ELISA:<br>Sandwich:<br>In a sandwich ELISA (assuming 100 ?L/well), a concentration of 1.0-2.0 ?g/mL of this antibody will detect at least 1000 pg/mL of recombinant Human IP-10 when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Human IP-10 (38-252) as the detection antibody at a concentration of approximately 0.25 ?g/mL.<br><br>Western Blot:<br><br>To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ?g/mL (non-reducing) or at a concentration of 1.0-2.0 ?g/mL (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: CXCL10
Clonality: Monoclonal
Clone: 3.3-1G1-2G7-2F6
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human

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