ELISA:<br>Sandwich:<br>To detect hIGF-BP1 by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IGF-BP1 (38-276) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.<br><br>Western Blot:<br><br>To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.
Type: Primary
Antigen: IGFBP1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human