Anti-IL21 Rabbit Polyclonal Antibody (Biotin)

Supplier: Prosci
38-213
10097-788EA 576.13 USD
10097-788
Anti-IL21 Rabbit Polyclonal Antibody (Biotin)
Antibodies
A novel cytokine related to IL2 and IL15 was recently identified and designated IL21. IL21 has been found to be a powerful growth factor for naive B cells. The receptor for IL21 (IL21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL21 signaling. Both IL21R and the gc are necessary for the IL21 function. IL21 and its receptor activate JAKSTAT signaling pathway. IL21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL21 plays a role in the proliferation and maturation of NK, B and T cell populations.

ELISA:<br>Direct:<br>To detect mIL-21 by direct ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21. <br><br>Sandwich<br>To detect mIL-21 by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine IL-21 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21. <br><br>Western Blot:<br>To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: Il21
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Rabbit
Isotype:
Reactivity: Mouse
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