Anti-WNT3A Rabbit Polyclonal Antibody

Supplier: Prosci
38-177
10098-336EA 576.13 USD
10098-336
Anti-WNT3A Rabbit Polyclonal Antibody
Antibodies
Wnt3a is a secreted ligand for members of the Frizzled family of receptors. It is one of the closely-related Wnt family and has multiple roles in development and cell fate.

ELISA:<br>Indirect:<br>To detect hWNT-3a by indirect ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.<br><br>Sandwich<br>To detect hWNT-3a by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.5 - 2.0 ?g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human WNT-3a as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. <br><br>Western Blot:<br>To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: WNT3A
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human
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