Anti-IGFBP3 Rabbit Polyclonal Antibody (Biotin)

Supplier: Prosci
38-153
10097-906EA 576.13 USD
10097-906
Anti-IGFBP3 Rabbit Polyclonal Antibody (Biotin)
Antibodies
Insulin like growth factor binding protein 3 (IGF-BP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGF-BP) and at least four low affinity binding proteins referred to as IGF-BP related proteins (IGFBPrP). The IGF-BP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGF-BP3 is fused to the signal peptide of CD33. Human IGF-BP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit.

ELISA:<br>Direct:<br>To detect hIGF-BP3 by direct ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3.<br><br>Sandwich<br>To detect hIGF-BP3 by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IGF-BP3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3. <br><br>Western Blot:<br>To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: IGFBP3
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human
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