Anti-IL20 Goat Polyclonal Antibody (Biotin)

Supplier: Prosci
38-133
10098-254EA 576.13 USD
10098-254
Anti-IL20 Goat Polyclonal Antibody (Biotin)
Antibodies
IL20 is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.

ELISA:<br>Direct:<br>To detect hIL-20 by direct ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-20.<br><br>Sandwich<br>To detect hIL-20 by sandwich ELISA (using 100 ?L/well antibody solution) a concentration of 0.25 - 1.0 ?g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-20 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-20. <br><br>Western Blot:<br>To detect hIL-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

The reconstituted antibody can temporarily be stored at 2-8°C. Long term storage at -20°C or below. Avoid repeated freeze-thaw cycles.

Type: Primary
Antigen: IL20
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Goat
Isotype:
Reactivity: Human
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