ZAP70(zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Zap70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the zap-70 gene. ZAP-70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.
Western Blot:1:500 - 1:2,000 <br>IHC(P):1:500 - 1:2,000<br>IHC(F):1:500 - 1:2,000<br>ELISA:Propose dilution 1:10,000.<br>Determining optimal working dilutions by titration test.
Ascitic fluid containing 0.03% sodium azide.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: ZAP70
Clonality: Monoclonal
Clone: 3D2A4
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human